Analysis for syphilis: RPR (RPR) test. The total result for syphilis Rpr test is negative

Syphilis RPR-test (03.13 or 03.14) Kit of reagents for the detection of antibodies to cardiolipin antigen in the flocculation reaction

Instructions on the preparation of a set of reagents for the detection of antibodies to cardiolipin antigen in the flocculation reaction syphilisRPR-test

Restratsіyne dedicated to No. FSR 2007/00406 dated July 16, 2007

1. RECOGNIZED Sifilis RPR test

For serodiagnostics of syphilis and control of the results of yogo treatment.

1. CHARACTERISTIC SET

2.1. The principle of dії

A test of the probing for the interaction of cardiolipin antigen with specific antibodies (reagins) in syrovatz or the plasma of a sick person for syphilis. To improve the visible signs of both positive and negative results of the reaction to the antigen, a particle is given. In case of manifestation in the sample of reagins, black aggregates are established, visible with an indelible eye. The flocculation reagins are not permeable, and the suspended suspension is uniform.

2.2. warehouse set

Reagent 1 RPR-antigen - aqueous 0.02% suspension of coarse particles of sensitized total cholesterol, cardiolipin, lecithin; a single suspension from gray to black colora, when taken to a black siege and a hole without barn nadosadovu ridin.

Reagent 2 is a positive control sample (K +) - inactivated, place antibodies to cardiolipin antigen in a titer not lower than 1: 4 (the titer is indicated in the passport of the skin seria); the prospect is unbarvna abo svitlo-zhovta ridina.

Reagent 3 negative control syrovat (K -) - inactive; prozora bezbarvna abo svitlo-zhovta ridina.

The delivery set also includes reaction cards.

The set is available in two complete sets:

set No. 1 (insurance coverage for 100 items);

set number 2 (insurance for 500 visas).

1. ANALYTICAL І DIAGNOSTIC CHARACTERISTICS SET

The number of insurance companies for carrying out 100 or 500 visas, including control studies.

1. ZAPOBIZHNI GO

The set is biologically safe, however, with prettier images, it turns out to be done with potential information materials.

1. HOBBY І MATERIALS

- Orbital shaker (whether of a brand with an amplitude of 10-20 mm).

- the micro-flow is automatic, with tips for 50 μl.

- Physiological breakdown - 0.9% breakdown of sodium chloride in purified water (distilled or deionized).

1. the analyzed vision

Native abo progrіta sirovatka abo plasma. It is not possible to vikoristovuvati hemolyzovanі, chіlesny or sprouted in a sprout. Syrovatki, intended for use, can be stored up to 7 dB at temperatures 2-8 ° С or up to 3 ms. at a temperature minus 20 o C.

1. ANALYZE Siphilis RPR-test

7.1. Preparatory education

All reagents must be vitrized 15-20 minutes before the ear is analyzed at room temperature.

The suspension of the RPR antigen is taken to a single stan.

7.2. analysis

without value

1. Apply 50 µl (one dots) of K +, K and pre-juvenile images to the midpoints of the disposable card (one spacer per teapot) with a micropipe. Place the line along the entire surface with the tip of the micropipette. For dermal vision, the vicoristovuvati okremiy tip.

1. Apply 20 µl of RPR antigen to all media. Place the cards on the platform of the orbital shaker and wrap them in the horizontal area 8 xv 3 x 180 rpm. Immediately to carry out an array of results.

Doslidzhennya To + i To it is necessary to carry out with the skin serіє udlіjuvanih srazkіv.

Invalid value (value for title)

It should be carried out only for a few moments, as they gave a positive reaction.

1. Prepare on single-use cards for serial two-way breeding of pre-cut images and K + - from 1: 2 to 1 1024. Vikoristovuvat card for skin exam.

With robots with cards, you can stick your fingers to the middle!

Apply 50 μl (one dots) of physiological range for all tests. Then, in the middle number 1, the first card to add 50 μl (one speck) of the first pre-juvenile sample and mix physiological discernment... Transfer from center # 1 50 µl (one speck) diluted 1: 2 at a time, transfer to center # 2 and mix. Repeat the procedure until the last dilution is removed (1 1024) in the middle No. 10. The vikoristovuchi tip of the micropipette, the droplets on all surfaces of the intermediate dilutions, collapsed from the greater dilution to the smallest.

Apply 20 µl of RPR antigen to all media. Place the cards on the platform of the orbital rotator and wrap in a horizontal area of ​​8 min at 180 rpm. Immediately, in order to carry out an array of results, fixing the K +.

Non-corrosive reagents for the first packaging are allowed to be taken in sealed vials at temperatures from 2 to 8 ° C until the end of the set.

Open packets with cards at room temperature.

1. RESTRACTION TA RESULTS OBLIQUE Syphilis RPR-test

without value

Positive reaction - in the sample, large, medium or other aggregates are visible.

Negative reaction - we see aggregates in the sample on the day.

Note: The presence in the sample of the reaction of a breakdown because of the irregularity of the loading when the visible aggregates are not familiar with the positive reaction

napivkіlkісne value

The results of the titration are also effective in the case of the correct quality.

The titre of antibodies to RPR-antigen is immediately involved in the rest of the dilution, in which it is removed positive reaction(Razr_znyayayutsya visible aggregates).

The titer of antibodies in a preliminarily vrakhoyuyut for the reason, but the titer of antibodies in K +, withdrawal in the reaction, is rendered from the value indicated in the passport, not more than on one dilution.

Note : The presence of reagins (antiyl to cardiolipin) in syrovattsi or plasma of humans can be a small fraction of a syphilis also in case of a number of diseases and physiological states in the body, such as infectious mononucleosis, Vagіtnіst, viral pneumonia, systemic chervonia vovchak, rheumatoid arthritis, Malar_ya, leprosy and іn. In connection with the whole set of positive results, recognized for the additional set "Cyphilis RPR-test", it is due to the additional set of specific methods of pre-screening, as well as for the test of treponemal antigens (it is recommended to test for the test systems).

1. TERMIN OF ADVANTAGES. UMOVI ZBERIGANNIA І TRANSPORTATION

method of value Flocculation (RPR antigen, which can be used in kits, modifying the VDRL antigen).

follow up material bloodshed

Accessible entrance to the booth

Nonspecific antiphospholipid (reaginic) test, a random analogue of the Wasserman reaction (RW), modification of the VDRL (Venereal Disease Research Laboratory) test.

RPR is a screening non-treponemal test that detects antibodies (reagents) of the IgG and IgM class to lipoid and lipoprotein-like material, which can be detected from the patient's ear cells for syphilis.

Antiphospholipid antibodies, which appear as a cym test, are found in 70 - 80% of patients with primary syphilis and in 100% of patients with secondary and early latent syphilis. In most cases, a positive RPR-reaction is delayed after 7 - 10 days after the appearance of the primary chancre, or after 3 - 5 days after the initial information. Titri is reduced by the second stage of syphilis. Closely 30% of patients with diagnosed syphilis become non-reactive according to the RPR test. Decrease in RPR titer in 4 and more times for 1 year after the therapy was carried out to confirm the effectiveness. In 90 - 98% of cases of syphilis elimination, the RPR test result becomes negative.

The test is not specific. In this case, it is possible to convince oneself of positive reactions. Antiponeal antibodies can be found not only in cases of syphilis of those who are often infected with trepidation, but in the case of development of non-negligible people who are attracted by hospitality and chronic feminine nature,

If a positive result in the RPR test is rejected, the patient is guilty of being a dermatovenerologist for repeated blood tests for a specific treponemal diagnostic test (in the INVITRO laboratory - a test, antibodies to Treponema pallidum antigens to IgG / antigen antibodies)

Ridky negative results in the RPR reaction ("Prozon" effect with even higher titers of reaginovyh antibodies) can be included in the one-hour test for syphilis IPA IgG / IgM. Tsi two doslidzhennya є vzaєmodopovnyuchim; kombinovane vikoristannya RPR tests and IPA-IgG / IgM tests represent a shortened version of screening dosage for detecting or detecting syphilis at all stages.

Features of infection. Syphilis - chronic Infectious disease, Scho is characterized by multisystemic organisms. Syphilis abnormalities - Treponema pallidum - nasty in navkolishny midst, albeit kindly taken from biological biological materials (sperm, vaginal secretions, mucus, pus, etc.). Transmitted by statusvim, parenteral, pobutovim, transplacental routes. Increase the risk of infection with other infections, which can be transmitted by a body path: herpes, chlamydiasis, papilomavirus infections and other infections. Bleed treponema is less than antigens, which can be used to summarize antigens. One of them is analogous to cardiolipin, which allows vikoristovuvati the rest for the development of immunity up to treponema.

Infection from sick people is possible in any period of syphilis. Most infectious and primary and secondary syphilis with active manifestations on the skin and mucous membranes. Bleed treponema penetrates through microscopic shkіri or mucous in lymphatic vessels, sometimes in lymphatic universities. The distance from the animal to the organs is widespread and can be found in all biological centers (slugs, breast milk, sperm, etc.).

With a classic interruption Incubation period 3 - 4 months, primary seronegative - 1 month, later primary seropositive - 1 month, sometimes second period - 2 - 4 rocks, long third period. In the first period of the disease, there is a hard chancre (painless movement of a disease with a cracked bottom in the moment of penetration of treponemia), which is supervised by regional lymphangitis and lymphadenitis. In the end of the first period, the chancre heals on its own, and lymphadenitis goes into polyadenitis and takes up to five months.

Perceiving generalized visipannya є a familiar ear of the secondary period. Secondary symptoms are sickly (for 1.5 - 2 months of skin disease) and self-awareness. It can be represented by pustular, papulosic, pustular syphilides, syphilic alopecia (oblastinia) and syphilic leukoderma ("namisto Veneri"). On the other side of the secondary syphilis, there is polyadenitis.

The emergence of theoretical syphilides (humps and humi) marks the ear of the tretine period, but 40% of the noncautious and disingenuous have been diagnosed with ailments. Chvili of the third period of distribution is more trivial (in some bagatory) periods of latent exclusion of infection. In urazhennyh organs and tissues, destructive changes are found. In theoretical sithilida, there is little treponema to take revenge on, so the stench is practically not contagious. The tension of immunity is falling (i.e., there is a change in the number of blidich treponems), which is becoming newer of information (resuperinfection).

Transfer of syphilis to the offspring of the most imovirna in the first three rocks. As a result, there are changes in wikis (for 12 - 16 years), neoplasms, early and early growths of syphilis. Cutting to the fetus is best on the 5th month of vaginosity and before the hour is low. Manifest an early congenital syphilis at once to the public and analogous manifestations of secondary syphilis. Symptoms of congenital congenital syphilis are observed in 5 - 17 years and similar manifestations of tertiary syphilis. The Hutchinson's tradition (Hutchinson's teeth, parenchymal keratitis, labyrinthine deafness) is a sign of madness.

Diagnosis of syphilis is guilty of being confirmed by laboratory predictions, however, in some cases there may be priming, unaffected by negative results of serological reactions.

Especially significant Laboratory diagnostics syphilisu maє in the offensive categories obstructed.

Analyze for syphilis rocking, stagnation without the help of methods for detecting a child. With the development of pathology, a large number of specific signs In addition, with the aim of establishing an accurate diagnosis of ailing people, it is necessary to work around in a complex manner. Zagalny analiz blood is not very informative at all, it is not vicious when diagnosed with a given venereal infection.

See doslіdzhen and biomaterialіv for analysis

For the detection of ailments, vikoristovuyu use different methods and biomaterials. At the early stages of syphilis, start for an additional bacterioscopic test. Learn to do it with a microscope. The attachment is allowed to wiggle with stitches. It is better to carry out serological analyzes of blood. Zavdyaki їm, antigens and antigens to syphilis can be detected in the samples.

Ways of assigning a status of infection to 2 categories:

• Straight, pathogenic microorganism emerge. Before them, there are: dark-field microscopy, RIT-analysis (information about rabbits in biomaterial for pre-adulthood), the PLR ​​method - a polymerase lanceol reaction (from the other side to know the genetic elements).
• Indirect (serological) allow the antigen to the pathogen. stink immune system, Yak is a common reaction to the infection.

Serological methods of subdivision into 2 categories: treponemal and non-treponemal.

• Non-treponemni, which include: test with toluidine worm, analysis of RSK, RPR-test, blood monitoring by express-method of RMP.
• Treponemni, which include: immunoblotting, RSK-test, РІТ-analysis, advanced RIF, RPGA-test, IFA-analysis.

Informativeness of tests for infection of the disease. Most often it is necessary to check the main types of analyzes for syphilis, before those introduced serological methods.

Biomaterial for doslіgennya

I will find that it is a pathogen, like a viglyad, like a spiral and viclikal syphilis, take samples:

• venous blood;
• liquor (secret from the spinal canal);
• vm_st lymphatic universities;
• fabrics and bends.

If it is necessary to carry out a test for the occurrence of syphilis, they do not build shelter only from the vein, but from the finger. On the vibir biomaterial and the way to keep up with the severity of the infectious diseases and the equipment of the diagnostic center.

direct dosage

Let's overconflict the proof of syphilis є detection of infections with a microscope. In this way, the pathogen can be identified in 8 patients with 10. Negative result in 2 patients were too overwhelmed not to talk about those who didn’t smell.

Beforehand, hesitating at the first and second stage (stage) of becoming ill, for those who are characterized by the appearance of hanging on the shkiri and siphiloma (virazok) on epithelial fabrics or slimy shells. In vid_lennya s in the midst of an infection, there are pathogens that are susceptible to venereal disease.

More precisely, the folding test can cope with the values ​​of treponemes, it is recognized as RIF - the reaction of immunofluorescence. The reader should be filled with fluorescent antibodies in front of the front. The spoluki, built up, are glued together with bacteria. Look out from the microscope at a glance, in case of an infected laboratory assistant to back up blistering spikes.

Test for early diagnosis of ailments. What is more, less than the sensitivity of diagnostic methods. In addition, there is a fall in the course of the crumpling of the hang-up and the use of antiseptic methods and of the patients who have gone through the treatment. The success rate is given to positively negative and positively positive results.

RIT-analysis is a high-precision method for detecting syphilis. When the test is carried out, the results are brought to check. Until quietly, when the infected rabbit does not show signs of infection. The test stagnates even more rarely, do not marvel at those who are super accurate.

For help polymerase lantsyugovy reaction on syphilis, there are genetic elements of pathogens. One half of the PLR ​​is a road trip.

not treponemni testimony

Such blood analyzes will help to identify antibodies, which are shown in the case of cardiolipin - spoluka, which should zalny budova membranes of pathogens.

Wasserman reaction (PB or RW)

The most recent reports on syphilis are affected by Wasserman's reaction. RV enter into the category of reactions of complement linking (RAC). The new methods of the RSK may be of the historical importance from the traditional RW. Alle, a stench will come to be known, like the “reaction of Wasserman” before the understanding.

The immune system, the synthesis of antibodies (markers) in response to the invasion of treponemes. Їх appear when analyzing blood for syphilis by the Wasserman reaction method. The positive result of RW confirmed the information about the situation.

Reaction to hemolysis - index of RV analysis. When there is a combination of 2 substances: hemolytic syrovat and erythrocytes of a ram. Syrovatku shy, immunizumo rabbit with ram erythrocytes. The activity of biological origin is reduced by the progress of progress.

Indicators of RV are due to the fact that hemolysis is gone. A sample of pure markers will show hemolysis. With a whole reaction to antigens is uncomfortable. The complement is vitrized for interaction with sheep erythrocytes. If it is a marker, the compliment enters into a reaction with antigens. In general, hemolysis does not contradict.

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Yak with syphilis viglyadaє hard chancre?

Components for RW are viewed in rivniy kilkost. Zrazok, how to take revenge on the sirovatka, antigen and complement, program. Add sheep erythrocytes and syrovat to the sample. Trim at a temperature of 37 degrees, as long as the hemolysis does not appear in the control test, which replaces the antigen to avenge the fizrozchin.

Antigens are prepared for carrying out RV. On the packaging, the titration and technology of breeding should be included. A positive RW result will be indicated by crosses. Prepare the test results and mark the next rank:

• ++++ - the most positive (hemolysis zatrimavsya);
• +++ - positive (hemolysis is very noticeable);
• ++ - weakly positive (hemolysis is often soaked);
• + - total (hemolysis was insignificant).

With negative RV, hemolysis increases in all patients. Ale in deyakie vypadkah take hybnopositive tribute. It is also considered, if cardiolipin is added to the storehouse. Due to the mechanisms not to produce markers up to "normal" cardiolipin.

However, it is clear that the situation is wrong. Positive RW appears in uninfected people. It’s so good, like the patient is transferred grave ailment, Viklikanu viruses (pneumonia, malaria, tuberculosis, liver and blood pathologies). Positive RV buvaє among the vagitniki. The cetus was paused, that the immunity is overwhelmingly weakening.

As soon as the patient is diagnosed with pseudopositive syphilis analysis, the patient will be tested beforehand. The problem is that it’s uncomfortable to get infected for one single clinical-laboratory test. A part of the doslidzhen is not true indicators, both negative and positive.

Recognize the valid data of the supplementary aid for the analysis of syphilis. Yomu zavdyaki vstanovlyuyut true diagnosis: to bring the infection or turn it on. In addition, the extensions of the test allow the development of infections, the inclusion of unnecessary therapy.

RSK i RMP

Carry out a cleanup on syphilis, the traditional reaction of Wasserman, to get rid of it in the region. Replace it with the method of RSK. The analysis gives a positive result after 2 months of information. In the case of a second form, there is a positive growth rate of 100%.

Method of microprecipitation (RMP) - continued with the mechanism of analogous reaction of Wasserman. The technique is simple for the Viconanne. Vaughn shvidko carried out. For further development, shelter for syphilis is created with a finger. The technique gives a positive result after 30 days from the moment the syphiloma appears. The pardons are not included in the trial. It is extremely positive to recognize on aphids: infection, pneumonia, infarction, insult, and intoxication have become aggravated.

Before pardoning the tests, produce:

• tuberculosis;
• Benier-Beck-Shauman's ailment;
• rheumatoid illness;
• diabet;
• cirrhosis;
• brucellosis;
• leptospirosis;
• mononucleosis.

Having detected a summation of the analysis for syphilis, weakened treponemni until the end of the day. The stench will help you to clarify the diagnosis.

RPR i test with toluidinovym wormon

The method of plasma reagins (RPR) is a Chergov analogue of the Wasserman reaction. Yogo to stop, if you need:

• screening asymptomatic individuals;
• pid hardness syphilis;
• donor donor shelter.

Test with toluidinovym wormonim, yak and RPR, to conduct, to assess dynamics drug therapy... Їx indicators fall when ailments enter, and increase, if the pathology recurs.

treponemni dosljennya

Conduct highly effective analyzes, vikoristovuchi treponemal antigens. Їx timid, if:

• a positive result with the RMP method is recognized;
• it is necessary to recognize the priesthood of the date, which is determined during the screening tests;
• take the development of syphilis;
• it is necessary to diagnose it with a stream of infection;
• need to carry out retrospective diagnostics.

Test RIF і РІТ

In the case of the infirm, the patients with treponemal tests give positive results for the next hour. Behind him you can judge about the level of efficiency of treatment. РІТ і РІФ - sympathetic tests. The managers їm will get the authentic data. Quite the analysis of labor, the stench vimagayut decent time-clock vitrates, the manifestation of progressive possession. Їх zdatnі conduct qualified medical specialists.

RIF analysis for syphilis, positive data will be received after 2 months of information. Negative parameters confirm health conditions. Positive - allow to let it go, but the person is informed.

Perform RIT if the microprecipitation reaction is positive. Such an analysis of blood for syphilis is additional help due to the fact that there is evidence of infection. The test is hypersensitive, it accurately detects infections of the patient or health. Allegedly, there is a valid date only after 3 months of penetration of treponemes into the body.

immunoblotting method

We carry out immunoblotting prior to precise tests. Such an analysis of blood in case of syphilis is rare. Yogo zasosovuyt at regular newborn children. Wine is not suitable for express testing. Positive results and feedback from records. Їх nabagato earlier yes the method of microprecipitation.

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What poznachayut whine in the analysis for syphilis, how robiti, how about 4?

ІFA і RPGA

Prior to informative advanced methods of admission to the test of the IFA and RPGA. From їkh help to carry out express diagnostics. Laboratory assistants are able to assess the magnificence of the number of such analyzes. The managers їm vdaєatsya to establish an accurate diagnosis.

RPHA analysis for syphilis positive after 30 days after the penetration of the child into the body. I can help you diagnose the primary infection, if there are viral and visceral infections. Youmu workers will be thrown into a run vrodzheni formi pathology. Alle to carry out it spіlno with non-treponemal and treponemal tests. Comprehensive diagnostics are not indicative of the reliability of the results. Try not to accurately bring the test to the presence of venereal infection.

IPA analysis is positive after 21 days from the moment of infection. The test only gives good results. Smell z'yavlyayutsya with systemic pathologies, damaged communication processes. Їх the effectiveness of the sumnіvna in the child, as was born from the information mother.

Abductions, obsessed with serological methods of advancement, became the reason for the emergence of progressive diagnostic methods. Gas chromatography and mass spectrometry do not give good results. One pereshkoda for їkh mass victoriannya is a road.

diagnostic algorithm

• In case of known syphilis in the first phase (up to 60 days at the time of the infirmity), the beetle will appear on dark aphids, or vikoristnoy for fluorescent antibodies.
• There is also pathology in the primary, secondary and secondary forms of stagnation of RMP and IFA. Confirm the results of additional assistance with RPHA blood analysis for syphilis.
• In case of recurrences of secondary infections, analyze the appearance of viral infections. Zrazkіv vityagayut zbudnikіv, vivchayut їkh behind an additional microscopy.
• If the disease enters the tretine phase, 1/3 of the patients have RMP negative. At the same time, the results of the IFA and RPGA are positive. However, the stench is not expected to be used for the third period, but it will last when people have had an infection before. weakly positive test- a proof of a general lіkuvannya, and not a development of the third phase.
• Pidtverzhuyuchi congenital syphilis, blood analysis is taken from the mother and dumb. Read the data on the RMP tests. Vrahovyat those who have a baby ІFA and RPGA positive. Pidtverzhuyut diagnosis, vikoristovuchi method of immunoblotting.

Syphilis, as if it were a systemic pathology, hostile to all organism. To that, obstezhenya on new pass at vagіtnostі, before an abortion. For the patients, to roam RMP, IFA, RPGA.

Yak zdati analiz

A doctor-venereologist guided patients for analysis. Private laboratories to check the announcements of the messages on the syphilis for the clients of the client. For the test, they do not need the direction of the liquor.

Rules for conducting an admission:

• Shelter in the laboratory should be taken on an empty slunker (for a procedure). Before the analysis, it is allowed to drink only water.
• For 2, finish off the hardening of the hardened: grub with fatty hedgehog and vzhivat alcohol.
• Take blood from your finger or veins.
• How many words are you talking about? Zazvychay no more dobie. Deciphering of analyzes for syphilis can be obtained from laboratory technicians of any drug.
• How much is a valid test? After 3 months of analysis and analysis will consume power. Їx create knowledge.

As the interpretation of the analysis shows that the test is positive, it is necessary to inform the venereologist, in order to determine the detailed information for accurate confirmation of the diagnosis and confirmation of the scheme of the necessary addiction.

Spinal test vm_stu

The diagnosis of neurosyphilis should be made in order to get the liquor up to date. Such an analysis of shyness:

• people with a haunted form of infection;
• with symptoms of illness nervous system;
• asymptomatic, advanced neurosyphilis;
• We are sick with positive serological reactions.

Directed to the doslіdzhennya lіkvoru daє lіkar. Take a puncture from the spinal canal in 2 tubes. Cover the puncture with iodine, close it with a sterile servet. For the procedure of the patient, the patient must be treated in the post-regime for 2 days.

At 1 point, you can start with a small number of bottles, klitin, follow the meningit. In other samples, count antibodies to the origin of syphilis. To test the test: РВ, РМП, РІФ і РІБТ.

Fallen from that, there was a debris, there are 4 different types of liquor. Skin is applied to the structure of the nervous system. Likar diagnostics:

• Sudinniy neurosyphilis;
• syphilic meningitis;
• dorsal dryness and іnshe.

Moreover, the test results are used to judge the patient's performance.

Interpretation of tests - the owner of the doctor. Tilki vіn zdatniy zrobiti correct visnovka, if necessary, indicate additional observance, deliver an accurate diagnosis. It is not easy to self-diagnose in case of unfavorable systemic pathology. Pomilka in diagnostics means serioznі inheritance.

The RPR test for syphilis is a very quick analysis, not the reaction of Wasserman, if the offense is late, it may be possible to detect it, or simply because of the obviousness of this illness. RPR is a non-treponemal test, which is a good test for reactivity (antibodies), which indicates a transfer or a progressive syphilis.

The antibodies, which appear behind the aid of the given test, are present in about 80 people with ailments in the primary form, and also practically in 100% of them it is quiet, who suffers in the secondary form or early latent syphilis. The RPR test will show positive results for the first time, as a rule, by stretching the tension in order for it to be a hard chancre. To be able to analyze the building up, it will take about 20-35 days from the moment when contact with the nose was made.

Indicators of the given dough grow only on the ear of the ear. Correcting at another stage, the analysis will show all the lesser number of antibodies, and the third ailment, in which a third syphilis is revealed, is not reactive according to the RPR test. Estimate the RPR titer in case of illnesses: a decrease in its indicators in some cases or more, as a result of therapy, to inform about efficiency. It is almost 100% quiet, when it comes to syphilis, the RPR test is passed with a negative result.

Analysis for syphilis RPR is not specific. This does not itself include the possibility of verifying positive results. In addition, antibodies, such as evolving analyzes, are created to appear in the organisms of people without the participation of treponemes, for example, in cases of autoimmune diseases. As soon as the RPR syphilis is reached, it will still be shown, the patient in the general order will be given additional conditions.

Danish analysis is carried out not earlier, not in 8 years after the end of the day. For a new understanding, the following is shown:

• Defeat at adolescence on venereal disease (manifestation of signs, vidomosti about contact with ailments);
• Diagnostics for detecting latent form of illness;
• Devotion of people, as they viyavili bazhannya to build donor shelter;
• Screening.

It is not guilty of a drive to diagnose illnesses, especially when the stage of development of such a situation, when the signs are visible, is not to blame for any circumstances. Positive results and reliable only in case of confirmation of treponemal delays.

Vasya, it means that you got a drink. Antibiotics for help.

rpr 1: 8 what does it mean?

www.zppp.saharniy-diabet.com

Syphilis RPR (Rapid Plasma Reagin - anticardiolepin test)

Syphilis RPR (Rapid Plasma Reagin - anticardial test) is a test for diagnostics of syphilis, which is found in the blood of antibodies to the singular components of the treponemia, the child of sickness.

Treponema Treponema pallidum is even less small, so it is impossible to virostitize "in the test" (on lively middlings), but rather to poke it in a light microscope. The method of detecting antibodies to the components of treponemia in the blood (serologic dosage) is the main one for diagnosing syphilis.

Pislya іnfіkuvannya bіdyu treponema organism viroblyaє two types of antibodies, some of which are straightforward to її development: specific and nonspecific. Specific antibodies (anti-treponemal) against treponemia itself, and nonspecific (anti-cardioleptic) - against the components of cells, which tend to run when affected by treponema, even if there is some blood

Analysis of "Syphilis RPR" reveals nonspecific antibodies of IgG and IgM classes to the last components. There are recommendations for screening the diagnosis, for the primary detection of infected individuals, as well as for the control of patients with syphilis.

Anticardial antibodies, which appear at a given time, appear in 70-80% of patients with primary syphilis. With secondary syphilis, it is 100% vypadkiv. A positive RPR reaction is more likely to occur in patients with syphilis even after a period of time when a solid chancre appears (after 4-5 tendencies at the moment of being inflicted with a treponema).

Likuvannya syphilis assess as efficiently, as if by extending the fate instead of nonspecific antibodies in the blood, it will change in a number of times. After completing the course of treatment, the RPR-reaction becomes negative in the majority of patients (up to 90%).

RPR-reaction is nonspecific, it can be positive in case of other pathological processes in the body: autoimmune disease and ailments with good fabrics(A positive test is often found in case of systemic chervonia vovchak); with vaginosti; tuberculosis; painting; Infections, vicious rickets; with intravenous drug administration; VIL-Infections; reddish diabetics; іnshikh zhvoryuvannyakh, scho wiklikayutsya treponemes - frambesia.

Shown to the analysis

Screening of vaginal women.

Screening of special features with non-contact article contacts.

Preoperative training.

Donation.

Diagnosis of syphilis in case of manifestation specific symptoms, In the first place with chancre on the body organs, or in the mouth empty.

Preparing until the end of the day

From the rest of the reception until the blood was taken, the hour's time was guilty for more than eight years.

First of all, you should be able to eat fatty foods, do not take alcoholic beverages.

For 1 year before taking blood for analysis, it is not possible to burn.

Shelter for a long time is being created in the heart of the heart, to drink tea or kava.

It is permissible to drink water.

Material for doslіgennya

Venous roof.

Interpretation of results

Analysis є yakisnim. Even though anticardial antibodies are not detected, the type is marked "negative".

Whenever anticardial antibodies appear, they appear “positively”.

Norm: negative opinion.

1. Visibility of infection with treponema (Treponema pallidum).
2. Early pervinny syphilis, if the antibodies have not yet been established.
3. Pizniy third syphilis.

Positive opinion.

1. Primary seropositive syphilis (infection occurred close to 1-3 months).
2. Secondary seropositive syphilis.
3. Stan pislya lykuvannya syphilisu (stretching out one rock).
4. Hibopositive reaction:

• Systemic incapacitation with the resultant fabric.
• Viability.
• Tuberculosis.
• Malyar_ya.
• Drug addiction.
• Diabetes mellitus.
• Viral hepatitis.
• Gout.
• Frambesia.

medportal.org

Deciphering the analysis for syphilis: weakly positive, pardonable, hibopositive result

syphilis є serious ills high grade contagiousness. For the detection of ailments, an analysis of blood (venous and capillary) should be performed, as well as the spinal cord ridina... Decryption of the analysis for syphilis is carried out by an eligible drug. The patient can self-indulge in the intelligence of the actions of the analysis, or the residual visibility about the manifestation of the day-to-day illness is to blame for the quality of the qualifications of the license. Mozhlivy hibnopositive or pomilkovo negative analysis for syphilis.

If it is necessary to turn to the lykar

Dovgy hour syphilis buv to poor people, I like to go to love. Modern medicine can be used in its own way in all its own way, in order to increase the disease. If the diagnosis is made and the disease is detected, it will be easier to get through the treatment. Infection with syphilis occurs not only with statutory contact, but also with viciousness of some and quiet by-side objects with ailments ( Toothbrush, Rushnik, kitchen utensils, etc.). To that, periodically carrying out an express analysis of blood for syphilis is recommended for skin people.

When infected, there is an increase in lymphatic universities in the groin area, and in the area of ​​the company and state organs. In case of the development of the first symptoms of ailments, they inadvertently turn up to the drug. Obstezhennya can be anonymous directly from a gynecologist, urologist, proctologist, venereologist or an individual therapist. After passing the test, it turns out for a deciphering analysis for syphilis to a drug.

quality assurance

Often, when medical examinations are carried out, a large number of laboratory analyzes can be indicated, including a test for syphilis. Do not sprymati such a straight line, like a dose of ailment. In the spheres of suspicious life, there is a need for a clue about the visibility of ailing.

• planning a family
• Decorated in a gurtozhitok
• Admission to the workmanship for medical professionals, workers of the community health service and in.
• Organ donation or blood
• Patients who lead more actively status of life
• frankness key symptoms
• End of treatment for syphilis

In the capacity of the primary dosage, as a rule, one of the nonspecific (non-treponemal) analyzes is recognized. The veracity of such tests is occasionally inconsistent and the patient can be positive result... In this case, it will be indicated to repeat the diagnosis from the specific (treponemal) test. Positive or negative analysis is guilty of being examined by the doctor.

Preparation before dough

Before donating blood from a finger or veins for laboratory dosage it is necessary to follow these rules, so that the analysis is as reliable as possible. For 8-12 years before the collection of blood, it is not necessary to live, tea, or kava. With a stretch, it is not recommended to live food, grease, grease, salted or smoked products until the laboratory is opened. Antibiotics and other drugs can also be tested. About all the accepted words of the doctor. If you can, I will recommend you to complete the analysis from 1 to 1 decile. A blood sample can be taken in a private laboratory, a district clinic or a doctor's office.

Anyone who has a vicarist will have a sterile possession of that disposable mittens.

Express-analysis on syphilis can be generated independently in the home mind. Pharmacies report instruction Russian language. The result of the vidomy test is already after 10 minutes. One small heart on indicators є negative analysis for syphilis, two small women - positive. The veracity of such tests is insufficiently visible and cannot serve as a confirmation of diagnosis.

Yak intelligence is the result of nonspecific clarification

Patients often feel uncomfortable with the test. Build shelter and could not break the deciphering of analyzes on syphilis independently, viciously, unacceptably. Decryption of blood analysis vimag medical education and the general qualifications of the doctor, as well as all the factors that are added to the result. How can a patient independently read the results of his analysis for syphilis? Having repaired the sound of the laboratory, it is possible to break the problems of the visnovka, or even confirm or simply diagnose the problem.

The test with toluidine worm is designated not for diagnostics, but for reversing the effectiveness of treating ailments. The number of antibodies has been changed beforehand with the previous analysis. As soon as the figure has changed, then the process is successful. The analysis is carried out in the process of testing the number of development for the signs of the drug. After 3 months after the completion of the procedures, a control test is carried out.

Non-treponemal tests (RSKk, RMP and RPR) are often used in medical examinations and as express diagnostics. There are many options in terms of results. It is simple to decode їх to finish:

• "-" negative result
• "+", "1 +") or "++", "2 +" weakly positive analysis
• "+++", "3+" or "++++", "4+" positive analysis for syphilis

Whether the result may be a positive or negative reaction to syphilis. With the appearance of clinical symptoms and severe sexual contacts, a negative result can be taken by a doctor, as a result. Positive reaction urge to change for an additional treponemal test.

Specific Dosage Results

Treponemal tests are expensive to cost for non-treponemous people. There are many types of analyzes that can be used for diagnostics of syphilis: RSKt, RIBT, RIF, RPGA, IFA and immunoblotting). One of the exact specific dosages is IBT-analysis. The test result can be presented in the laboratory as a percentage.

• 20% responded to a negative result ("-")
• 21-30% of total analysis ("++" or "2 +")
• 31-50% weakly positive ("+++", "3+")
• 51% and more results in a positive result

Immunoblotting is one of the most advanced and precise methods for diagnosing illness. Zvvychay to be recognized in order to confirm whether or not to obtain the results of the first release. The appearance in the blood of antibodies to the type of IgG and IgM is indicated by men. The test results are deciphered in accordance with the non-treponemal test.

Iakshcho offending the result of negativity, the patient is healthy, or the infected person is on the first development. The offense of a positive result indicates the manifestation of syphilis chi, possibly, autoimmune illness.

A positive immunoblotting test for negative non-treponemal illnesses means the presence of syphilis, an autoimmune or oncological illness.

There may also be a positive reaction in the presence of those who are present. Negative immunoblotting test for positive non-treponemal illnesses means the incidence of illness.

Reliability of analyzes

Determine whether the result of the pardon test is correct. When deciphering analyzes for syphilis, special respect for the next factors that come to lie in the patient. You can also have a pardon for a laboratory assistant who can carry out a follow-up or a patient, if it is wrong to prepare for taking blood, for not giving the doctor truthful information about himself. A merciful positive result is possible with the introduction of such factors:

• Type of diabetes
• Reveal in the blood of narcotic words
• Alcohol sp'yaninnya
• Ingestion of infectious type (cyr, hepatitis, mononucleosis, etc.)
• kindly abo evil new
• Ailment heart
• Accept antibiotics for recent fragmentation
• Autoimmune disease (chervoniy vovchak, rheumatoid arthritis, etc.)
• availability
• Vzhivannya fatty, greasy or salty їzhі reach out to the blood fence

Fallen from the stage of becoming ill, deyak_ father cannot be found ill. So, Wasserman's reaction (RSKt, і RSKk) is carried out only after 3-4 days if the infection is 100%, with the presence of a third-party syphilis, the reliability of the warehouse is 75%. For diagnostics of the early stages of illness, docillary test for the IFA test. Analysis is an enzyme immunoassay for high sensitiveness before antiit. The reliability of the result is close to 100%, it is highly positive result in case of the manifestation of other illnesses.

Negative results of analyzes on venereal disease mean that the person is healthy. Summarized analysis on syphilis production before re-rounding. As well as the presence of factories, which could be inserted into the bag, for example, the manifestation of those who were ill, could change the parameters of the test. A positive result of the analysis for syphilis is not generally a drive for panika. For the additional help of a medicinal inflow of ailment, you can get more vilikuvan. However, slid pam'yatati, scho get sick on early stage nagato is more beautiful to love.

krov.expert

RPR test

The Danish test even knows antiphospholipid antibodies in more than 75% of people who may have primary syphilis, and it is practically impossible in individuals who have tuberculosis with secondary syphilis. I will show practice, in about 30% of ailments on syphilis on to a different stage the manifestation of the disease by these test is not confirmed.

A positive test result can be recognized as early as on the 7-10th day when the first test (chancre) is detected on the only sick person and after 3 to 5 types of infection are diagnosed with syphilis.

The process of carrying out the consultation

preparation

before the given doslіdzhennya you need to go through the steps of preparation:

• before the analysis, it is carried out from the wound and to the heart, the patient is recommended to complete the test in 8 years (even more beautifully in 12 years) before the test;
• implantation of juices, tea, kawi, alcoholic beverages;
• 30 minutes before the cob of the analysis of the patient, ask to ask to go out of the house.

RPR test

The process of carrying out the given test has two main stages:

• taking blood from the patient's vein;
• The addition of cardiolipin antigen to the bloodstream, as a result of which the immune reaction of the precipitation to the antigen and antigen complex, which, as a rule, is low in dark color.

Pratsyu vin for additional value of anti-cardiolipin. cardiolipin- a special block that appears in the membranes of bacteria and mitochondria.

syphilis – ailment infection, Scheduled to ZPSSH (zapvoryuvannya, scho transmitted by a state road). Syphilis є to systemic discomforts, invitations to enmity, be it fabrics or organisms. Perebіg syphilis є chronic, without sickness lіkuvannya can be trivial, step by step organism.

Most often syphilis is transmitted during unprotected sex. Ailments can be transmitted by a vertical path, tobto. Nebezpeka syphilis in the fact that the next hour can be ill. Protest, the infectious process in the whole hour does not fall back, shkodi people are screaming.

So also RPR analysis for syphilis?

The whole analysis is a more accurate and accurate version of Wasserman's reaction. If a child becomes ill with a child’s organism, it’s from them that the material is produced. RPR analysis of conjugation for reagin detection. react- special antibodies to lipids.

If the RPR analysis for syphilis is indicated?

Such an analysis is indicated in case of obstezheniye, as well as a dose for syphilis. Likar can indicate RPR, please specify, what is the lack of syphilis. Reactions RPR are used to monitor potential donors, and an analysis is taken during screening tests. Krym, analysis of RPR is used to clarify the effectiveness of treatment. So, the test allows you to see it. As a result, the titer for RPR analysis decreases by a factor of 4, the therapy becomes effective.